Lymphoprep 500 ml

Isolate mononuclear cells from peripheral blood, cord blood, or bone marrow with Lymphoprep™—a cost-effective alternative to Ficoll-Paque™.

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Isolate mononuclear cells from peripheral blood, cord blood, or bone marrow with Lymphoprep™—a cost-effective alternative to Ficoll-Paque™.

Use this density gradient medium for rapid, simple, and reliable cell isolation from most blood samples obtained from normal individuals and patients. You can substitute Lymphoprep™ for Ficoll-Paque™ without changing your existing protocols and achieve similar cell purity and recovery rates.

This medium is fully compatible with both SepMate™ and RosetteSep™. Formulated with sodium diatrizoate (9.1% w/v) and polysaccharide (5.7% w/v), Lymphoprep™ has a density of 1.077 g/ml.CONTAINS• Sodium diatrizoate (9.1% w/v)

• Polysaccharide (5.7% w/v)
• Other ingredients

Density Gradient Media
CELL TYPE Mononuclear
Cells SPECIES Human
SAMPLE SOURCE Bone Marrow, Cord Blood, Whole Blood
APPLICATION Cell Isolation
BRAND Lymphoprep

Lymphoprep is a type of density gradient medium that is used in laboratory settings to separate cells or other biological particles based on their size and density. It is commonly used in a technique called density gradient centrifugation, which involves spinning a tube of Lymphoprep and other components at high speeds to create a gradient of increasing density. This gradient allows cells or other particles to be separated based on their density and size, with heavier or denser particles settling towards the bottom of the tube and lighter or less dense particles remaining towards the top.

Lymphoprep is specifically formulated to be used for the isolation and purification of lymphocytes, which are a type of white blood cell that plays an important role in the immune system. It is often used in research and diagnostic laboratories to isolate and study these cells, as well as to prepare samples for other analyses such as flow cytometry or gene expression analysis. Lymphoprep is a brand of density gradient medium that is made by Axis Shield, a company that specializes in supplying products and services to the life sciences industry.

Lymphoprep™ Isolation of human mononuclear cells

A simple and effective method for the isolation of mononuclear cells from human blood was reported by Dr. Arne Bøyum in 1968. For more than 35 years a commercial medium known as Lymphoprep™ has been widely used for isolating these cells.

Mononuclear cells (monocytes and lymphocytes) have a lower buoyant density than the erythrocytes and the polymorphonuclear (PMN) leukocytes (granulocytes). The vast majority of mononuclear cells have densities below 1.077 g/ml. These cells can therefore be isolated by centrifugation on an isoosmotic medium with a density of 1.077 g/ml, which allows the erythrocytes and the PMNs to sediment through the medium while retaining the mononuclear cells at the sample/medium interface.

Lymphoprep™ is a ready-made, sterile and endotoxin tested solution with the following specifications: 

  • Sodium diatrizoate: 9.1% (w/v) 
  • Polysaccharide: 5.7% (w/v) 
  • Density: 1.077 ± 0.001 g/ml 
  • Osmolality: 290 ± 15
  • mOsm Endotoxins: < 1.0 EU/ml